Open AccessA possible role for the yeast TATA-element-binding protein in DNA replication.
Author(s) -
Neal F. Lue,
R.D. Kornberg
Publication year - 1993
Publication title -
proceedings of the national academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.90.17.8018
Subject(s) - tata box binding protein , tata binding protein , dna replication , control of chromosome duplication , biology , origin recognition complex , tata box , origin of replication , eukaryotic dna replication , replication factor c , transcription (linguistics) , microbiology and biotechnology , promoter , dna , genetics , gene , gene expression , philosophy , linguistics
The TATA-element-binding protein (TBP) is involved in the initiation of transcription by all three eukaryotic RNA polymerases. The following observations implicate TBP in the initiation of DNA replication at yeast chromosomal origins as well: (i) Recombinant yeast TBP binds specifically to functionally important regions of many yeast replication origins in vitro. (ii) TBP-binding sites from RNA polymerase II promoters can activate defective replication origins in vivo. (iii) Point mutations in TBP-binding sites that diminish their affinity for TBP in vitro reduce their ability to support replication in vivo.
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