Open AccessHost genes that influence transposition in yeast: the abundance of a rare tRNA regulates Ty1 transposition frequency.
Author(s) -
Hua Xu,
Jef D. Boeke
Publication year - 1990
Publication title -
proceedings of the national academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.87.21.8360
Subject(s) - transposition (logic) , gene , biology , genetics , transfer rna , yeast , saccharomyces cerevisiae , rna , philosophy , linguistics
Genetic screening of a yeast genomic library in a high-copy-number vector identified the normally single copy tRNA(CCU Arg) gene as one of the genes and reduces Ty1 transposition frequency when overexpressed. Immunoblot analyses of Ty1-encoded proteins indicate an inverse correlation between the copy number of the tRNA gene and the production of the TYB protein. Thus, Ty1 transposition frequency is apparently regulated by the level of tRNA(CCU Arg) in yeast cells.
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