Open AccessLocalization of phosphatidylinositol signaling components in rat taste cells: role in bitter taste transduction.
Author(s) -
Paul M. Hwang,
Ajay Verma,
David S. Bredt,
S H Snyder
Publication year - 1990
Publication title -
proceedings of the national academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.87.19.7395
Subject(s) - phosphatidylinositol , inositol , inositol trisphosphate , taste , signal transduction , inositol trisphosphate receptor , diacylglycerol kinase , taste receptor , biochemistry , receptor , chemistry , phosphatidic acid , second messenger system , phospholipase c , taste bud , microbiology and biotechnology , biology , phospholipid , protein kinase c , membrane
To assess the role of phosphatidylinositol turnover in taste transduction we have visualized, in rat tongue, ATP-dependent endoplasmic reticular accumulation of 45Ca2+, inositol 1,4,5-trisphosphate receptor binding sites, and phosphatidylinositol turnover monitored by autoradiography of [3H]cytidine diphosphate diacylglycerol formed from [3H]cytidine. Accumulated 45Ca2+, inositol 1,4,5-trisphosphate receptors, and phosphatidylinositol turnover are selectively localized to apical areas of the taste buds of circumvallate papillae, which are associated with bitter taste. Further evidence for a role of phosphatidylinositol turnover in bitter taste is our observation of a rapid, selective increase in mass levels of inositol 1,4,5-trisphosphate elicited by low concentrations of denatonium, a potently bitter tastant.
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