English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Agrobacterium tumefaciens transfers the T-DNA portion of its Ti plasmid to the nuclear genome of plant cells. Upon cocultivation of A. tumefaciens strain A348 with regenerating tobacco leaf protoplasts, restriction endonuclease fragments of the T-DNA were generated that are consistent with double-stranded cleavage of the T-DNA at the border sequences. The T-DNA border cleavage was also induced by acetosyringone, a compound that induces many of the virulence genes. T-DNA cleavage did not occur in Agrobacterium strains harboring Tn3-HoHo1 insertions in the virA, -D, or -G genes. Insertion mutations in virB, -C, or -E did not have any effect on the T-DNA cleavage. Complementation of the mutations in virA, -D, or -G with cosmids containing the respective wild-type genes restored the T-DNA cleavage. Since virA and -G are essential in regulating the expression of other vir genes in response to plant signal molecules, the virD gene product(s) appear to mediate double-stranded T-DNA border cleavage.

Virulence genes A, G , and D mediate the double-stranded border cleavage of T-DNA from the Agrobacterium Ti plasmid