Open AccessAlternative use of 5' exons in the specification of Ly-5 isoforms distinguishing hematopoietic cell lineages.
Author(s) -
Yumiko Saga,
J S Tung,
F W Shen,
Edward A. Boyse
Publication year - 1987
Publication title -
proceedings of the national academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.84.15.5364
Subject(s) - complementary dna , biology , exon , microbiology and biotechnology , cdna library , alternative splicing , rna splicing , gene isoform , primer extension , genetics , gene , primer (cosmetics) , messenger rna , rna , chemistry , organic chemistry
Previous inferences that Ly-5 glycoprotein isoforms of murine hematopoietic cells are generated by alternative splicing of primary transcripts of a single Ly-5 gene are supported by the present study. A cDNA library was prepared from B cells by extension from primer representing a known T-cell cDNA sequence. Three different Ly-5 clones from this library included sequences missing in T-cell cDNA clones. From the constitution of cDNA clones and of the Ly-5 gene, and from S1 nuclease mapping, it is concluded that at least two exons, provisionally numbered Ex-6(B) and Ex-7(B), in the 5'-proximal region are mainly represented in mRNA of the B-cell lines examined but not of the T-cell lines examined. Also, exons 1 and 2 appear to be used alternatively in different species of B-cell mRNA and probably also in different species of T-cell mRNA.
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