Open AccessCloning and characterization of the high-affinity cAMP phosphodiesterase of Saccharomyces cerevisiae.
Author(s) -
Philip M. Sass,
Jeffrey Field,
Junichi Nikawa,
Tomohisa Toda,
Michael Wigler
Publication year - 1986
Publication title -
proceedings of the national academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.83.24.9303
Subject(s) - saccharomyces cerevisiae , phosphodiesterase , yeast , cyclase , adenylate kinase , mutant , biology , biochemistry , cloning (programming) , gene , camp dependent pathway , saccharomyces , microbiology and biotechnology , enzyme , adenylyl cyclase , programming language , computer science
A gene, PDE2, has been cloned from the yeast Saccharomyces cerevisiae that, when present in high copy, reverses the phenotypic effects of RAS2Val19, a mutant form of the RAS2 gene that renders yeast cells sensitive to heat shock and starvation. It has previously been shown that the RAS proteins are potent activators of yeast adenylate cyclase. We report here that PDE2 encodes a high-affinity cAMP phosphodiesterase that shares sequence homology with animal cell phosphodiesterases. These results therefore imply that the effects of RAS2Val19 are mediated through its changes in cAMP concentration.
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