Open AccessGeneration of a dominant 8-MDa deletion at the left terminus of vaccinia virus DNA.
Author(s) -
Eduardo Ortega Páez,
Shatha F. Dallo,
Mariano Estéban
Publication year - 1985
Publication title -
proceedings of the national academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.82.10.3365
Subject(s) - vaccinia , virus , mutant , biology , virology , infectivity , dna , virulence , modified vaccinia ankara , mutation , poxviridae , microbiology and biotechnology , gene , genetics , recombinant dna
Vaccinia virus mutants were obtained in high frequency from mouse Friend erythroleukemia (FEL) cells persistently infected with this virus, which contains a large (122-MDa) DNA. During long-term cell passages viral particles with deletions of the DNA are generated in FEL cells. These mutants have a major 8-MDa deletion starting between 2.2 and 3.2 MDa from the left terminus of the viral genome. More than half of the left end terminal repetition is deleted. These mutants have reduced infectivity compared to wild-type virus. The ease with which vaccinia virus mutants are obtained in FEL cells should provide a suitable system for generating mutants with other poxvirus and permit study of the genetic basis of virulence for this group of viruses.
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