Open AccessImmunohistochemical evidence for the coexistence of histidine decarboxylase-like and glutamate decarboxylase-like immunoreactivities in nerve cells of the magnocellular nucleus of the posterior hypothalamus of rats.
Author(s) -
Noriaki Takeda,
Shinobu Inagaki,
Sadao Shiosaka,
Yuzuru Taguchi,
Wolfgang H. Oertel,
M. Tohyama,
Tsuyoshi Watanabe,
Hiroshi Wada
Publication year - 1984
Publication title -
proceedings of the national academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.81.23.7647
Subject(s) - glutamate decarboxylase , histidine decarboxylase , nucleus , hypothalamus , magnocellular cell , immunohistochemistry , glutamate receptor , biology , endocrinology , medicine , chemistry , biochemistry , histidine , microbiology and biotechnology , enzyme , receptor
Immunohistochemical staining of alternate consecutive sections revealed numerous histidine decarboxylase (L-histidine carboxy-lyase, EC 4.1.1.22)-like immunoreactive neurons that also contained glutamate decarboxylase (L-glutamate 1-carboxy-lyase, EC 4.1.1.15)-like immunoreactive structures in the tuberal magnocellular nucleus, the caudal magnocellular nucleus, and the postmammillary caudal magnocellular nucleus of the posterior hypothalamus of rats. Furthermore, in immunohistochemical double-staining procedures, almost all neurons in the magnocellular nuclei had both histidine decarboxylase-like and glutamate decarboxylase-like immunoreactivities. These results suggest the coexistence of histamine and gamma-aminobutyric acid in single neurons in these nuclei.
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