Open AccessEnhanced recombination between lambda plac5 and F42lac: identification of cis- and trans-acting factors.
Author(s) -
H. Steven Seifert,
Ronald D. Porter
Publication year - 1984
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.81.23.7500
Subject(s) - operon , recombination , plasmid , lambda phage , escherichia coli , lambda , genetics , recombinant dna , biology , promoter , bacteriophage , microbiology and biotechnology , dna , gene , physics , gene expression , optics
Enhanced transductional recombination between specialized transducing phage lambda plac5 and plasmid F42lac depends on cis- and trans-acting factors. By constructing a series of recombinant molecules, the cis-acting site required for enhanced recombination has been identified as oriT, the origin of conjugational transfer of the F sex factor of Escherichia coli. The trans-acting factors are located in the promoter-proximal and the promoter-distal regions of the traY-to-traZ operon.