Open AccessConstruction of a retrovirus capable of transducing and expressing genes in multipotential embryonic cells.
Author(s) -
John L.R. Rubenstein,
JeanFrançois Nicolas,
F Jacob
Publication year - 1984
Publication title -
proceedings of the national academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.81.22.7137
Subject(s) - retrovirus , biology , gene , thymidine kinase , microbiology and biotechnology , transfection , recombinant virus , virology , viral vector , herpes simplex virus , recombinant dna , reporter gene , gene expression , cell culture , virus , genetics
Retroviral gene expression is inhibited in embryonal carcinoma (EC) cells. We have constructed a recombinant retroviral vector that is capable of expressing the neomycin-resistance (neo) gene in EC cells. The critical modification that permits expression of the neo gene is the insertion of a composite simian virus 40 early gene-herpes simplex virus type 1 thymidine kinase gene (SVtk) promoter 3' to the viral first intron and 5' to the neo gene. When the SVtk promoter is deleted, the recombinant retrovirus is either unable or extremely inefficient at expressing the neo gene in EC cells.
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