Open AccessChloroperoxidase generation of singlet Δ molecular oxygen observed directly by spectroscopy in the 1- to 1.6-μm region
Author(s) -
Ahsan U. Khan,
Peter A. Gebauer,
Lowell P. Hager
Publication year - 1983
Publication title -
proceedings of the national academy of sciences
Language(s) - English
Resource type - Journals
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.80.17.5195
Subject(s) - chemiluminescence , singlet oxygen , hydrogen peroxide , photochemistry , chemistry , oxygen , spectroscopy , peroxide , inorganic chemistry , organic chemistry , quantum mechanics , physics
The (0,0)1 Δg →3 Σ- g singlet molecular oxygen chemiluminescence emission from a biological reaction system, chloroperoxidase (EC 1.11.1.10) acting on hydrogen peroxide at low pH (phosphate buffer, pH 2.85) with Cl- as a cosubstrate, was recorded with an ultrasensitive IR spectrometer. The strong chemiluminescence emission peak observed at 1.30 μm provides clear evidence of the enzymatic generation of (excited) singlet oxygen from peroxide in this system.
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