Open AccessPrimary structure of chicken muscle pyruvate kinase mRNA.
Author(s) -
Nils Lönberg,
W Gilbert
Publication year - 1983
Publication title -
proceedings of the national academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.80.12.3661
Subject(s) - pyruvate kinase , complementary dna , messenger rna , biology , biochemistry , peptide sequence , protein primary structure , microbiology and biotechnology , amino acid , isozyme , coding region , pkm2 , enzyme , gene , glycolysis
We have determined the cDNA sequence corresponding to chicken muscle pyruvate kinase mRNA; the predicted coding region spans 529 amino acids and establishes the complete amino acid sequence for the vertebrate enzyme. We demonstrate that the level of mRNA for this enzyme is under developmental control and suggest a structural model for the protein kinase-mediated regulation of the mammalian liver isozyme. We report a method for the direct analysis of, and the preparation of cDNA probes from, mRNA which has been fractionated on methylmercury/agarose gels.
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