Open AccessInterferon-dependent induction of mRNA for the major histocompatibility antigens in human fibroblasts and lymphoblastoid cells.
Author(s) -
Marc Fellous,
Uri Nir,
David Wallach,
Gilles Merlin,
Menachem Rubinstein,
M Revel
Publication year - 1982
Publication title -
proceedings of the national academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.79.10.3082
Subject(s) - microbiology and biotechnology , lymphoblast , messenger rna , biology , interferon , complementary dna , fibroblast , human leukocyte antigen , major histocompatibility complex , histocompatibility , cell culture , cell , beta 2 microglobulin , gene , antigen , virology , immunology , biochemistry , genetics
In human cells treated with interferons, there is an increase in the amount of HLA-A,B,C and beta 2-microglobulin exposed on the cell surface. We have used a cloned HLA-A,B,C cDNA probe to demonstrate by molecular hybridization that this effect of interferon is preceded by a large increase in the amount of HLA mRNA in the cell. This effect was found in five different human cell lines, with purified leukocyte and fibroblast interferons. The increase in HLA mRNA is comparable in its kinetics and dose-response to the induction of (2'-5') oligo(A) synthetase mRNA by interferons. Therefore, interferons seem to activate at least two cellular genes which have different biochemical functions.
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