Open AccessProportion of fetal hemoglobin synthesis decreases during erythroid cell maturation.
Author(s) -
David H.K. Chui,
Shlu C. Wong,
Murray Enkin,
Michael M. Patterson,
Rowan Ives
Publication year - 1980
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.77.5.2757
Subject(s) - hemoglobin , fetal hemoglobin , erythropoietin , globin , fetus , in vitro , peripheral blood mononuclear cell , erythropoiesis , biology , andrology , immunology , cell culture , pregnancy , medicine , endocrinology , anemia , biochemistry , genetics
Peripheral blood mononuclear cells from pregnant and postpartum women were cultured in vitro with erythropoietin. Burst-forming unit (BFU-E)-derived erythroid colonies composed of immature erythroblasts with low hemoglobin contents were observed by day 8 of culture. By day 12 of culture, numerous BFU-E-derived erythroid colonies with high hemoglobin contents were present. The gamma/(gamma + beta) globin synthetic ratio was approximately 12% in the early cultures and 6% in the late cultures, indicating that the proportion of fetal hemoglobin synthesis decreases during erythroid cell maturation. These studies also reveal that the capacity fof fetal hemoglobin production by peripheral blood BFU-E in vitro is not altered during pregnancy.