Open AccessTranslation of animal virus RNA in the cytoplasm of a plant cell.
Author(s) -
Ewa Cairns,
H. H. Gschwender,
M Primke,
Michitaka Yamakawa,
Peter Traub,
Hans−Georg Schweiger
Publication year - 1978
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.75.11.5557
Subject(s) - acetabularia , cytoplast , cytoplasm , biology , rna , immunofluorescence , virus , translation (biology) , cell , microbiology and biotechnology , virology , messenger rna , genetics , gene , antibody
Isolated Acetabularia crenulata nuclei were injected with Mengo virus RNA solution and then implanted into anucleate posterior Acetabularia mediterranea cell fragments or fused with Acetabularia ryukyuensis cytoplasts. The injected animal virus RNA was actively translated in the plant cell cytoplasm. Mengo virus proteins were detected and localized in Acetabularia cytoplasts by use of an immunofluorescence method on the first to fifth day after injection.