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Fluorescence polarization and viscosities of membrane lipids of 3T3 cells.
Author(s) -
Pinhas Fuchs,
Abraham H. Parola,
Phillips W. Robbins,
E. R. Blout
Publication year - 1975
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.72.9.3351
Subject(s) - fluorescence anisotropy , fluorescence , membrane , polarization (electrochemistry) , 3t3 cells , biophysics , chemistry , lipid bilayer , virus , bilayer , pi , cell membrane , biology , biochemistry , virology , transfection , optics , gene , physics
Studies of the fluorescence polarization of 1,6-diphenyl-1,3,5-hexatriene in membranes of normal, transformed, and revertant 3T3 cells allowed estimation of microviscosities (eta) of the lipid bilayers of these cells. Fluorescence polarization values observed with normal cells were significantly lower than those observed with cells transformed either by polyoma virus or by simian virus 40. The values of fluorescence polarization obtained with revertant Py6R1 cells were lower than those obtained with the normal cells. The calculated microviscosities (eta) show a 50% increase upon transformation. Possible correlations between the bilayer viscosity and the mobility of the receptors in the membrane are discussed.

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