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Sucrose gradient analysis revealed an aldosterone-dependent increase in the incorporation of [(3)H]uridine into a nonmethylated, rapidly labeled RNA that sedimented at 9-12 S. This effect was antagonized by an anti-mineralocorticoid, spironolactone (SC 9420), and was not elicited either by a glucocorticoid, cortisol, or by the inactive isomer, 17-alpha-isoaldosterone. These findings are in accord with the inference that mRNA is induced by aldosterone during the latent period and that the induced mRNA mediates the action on sodium transport.

Kinetics of RNA Labeling in Toad Bladder Epithelium: Effects of Aldosterone and Related Steroids