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A bacteriophage λ strain has been constructed and a method developed by which DNA from potentially any source can be covalently inserted throughEco RI cohesive ends into the middle of the λ DNA. These hybrid DNAs can infect nonrestrictingEscherichia coli cells and can then propagate as plaque-forming phage. A unique feature of this λ strain is that extra DNA in the middle of its genome is required for plaque formation. A large number of such phages have been produced withE. coli DNA andDrosophila melanogaster DNA.

Viable Molecular Hybrids of Bacteriophage Lambda and Eukaryotic DNA