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Somatic cell hybrids between mouse cells deficient in thymidine kinase [ATP:thymidine 5'-phosphotransferase (EC 2.7.1.75)] and two different monkey cell lines transformed by an adeno 7-SV40 hybrid have been produced using both a semiselective and a double selective procedure. Concordant segregation of the expression of SV40 T antigen with a specific monkey chromosome has been observed in all the mouse-monkey hybrid clones examined. Subcloning of three SV40 T antigen positive hybrid clones resulted in their segregation into SV40 T antigen-positive and negative subclones. Positive correlation between the SV40 T antigen and the same monkey chromosome has been observed in all the subclones examined.

Chromosome Assignment of the T-Antigen Gene of Simian Virus 40 in African Green Monkey Cells Transformed by Adeno 7-SV40 Hybrid