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Guanosine tetraphosphate, recently discovered to mediate the regulatory relationship between protein synthesis and RNA accumulation in various bacteria, has been synthesized in vitro in large quantities and analyzed by natural-abundance (13)C nuclear magnetic resonance spectroscopy in order to confirm its structure and establish the positions of phosphate attachment. These studies have established its structure as guanosine 5'-diphosphate, 3'-diphosphate.

Guanosine 5′-Diphosphate, 3′-Diphosphate: Assignment of Structure by 13 C Nuclear Magnetic Resonance Spectroscopy

Guanosine 5′-Diphosphate, 3′-Diphosphate: Assignment of Structure by ¹³ C Nuclear Magnetic Resonance Spectroscopy