Open AccessHydrogen Evolution by a Chloroplast-Ferredoxin-Hydrogenase System
Author(s) -
John R. Benemann,
Jeffrey A. Berenson,
Nathan O. Kaplan,
Martin D. Kamen
Publication year - 1973
Publication title -
proceedings of the national academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.70.8.2317
Subject(s) - ferredoxin , hydrogenase , oxygen evolution , photosystem i , photochemistry , photosystem ii , chloroplast , photosynthesis , chemistry , water splitting , electron acceptor , hydrogen , photosystem , spinach , electron transport chain , biochemistry , catalysis , enzyme , photocatalysis , electrochemistry , organic chemistry , gene , electrode
Spinach chloroplast preparations were mixed with Clostridium kluyveri hydrogenase and ferredoxin. Hydrogen evolution could be measured in the light in the absence of any added electron donors. Inhibition of the water-splitting reaction or of photosystem II reduced the amount of H(2) evolved more than 95%, indicating that H(2)O was the electron donor in this reaction. The rates of H(2) evolution observed were up to 20% of those measured in the presence of an oxygen-consuming reaction or of photosystem I electron donors. These findings indicate that hydrogen evolution from water and sunlight by photosynthetic processes could be a method for solar energy conversion.
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