Open AccessIn Vivo Splicing of Protein: One Continuous Polypeptide from Two Independently Functioning Operons
Author(s) -
B. N. Apte,
David Zipser
Publication year - 1973
Publication title -
proceedings of the national academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.70.10.2969
Subject(s) - operon , gene , rna splicing , genetics , mutant , lac operon , biology , recombination , dissociation (chemistry) , enzyme , chemistry , biochemistry , plasmid , rna
Active-beta-galactosidase (EC 3.2.1.23) is often formed in rec(-) merozygotes containing a pair of mutations in the z gene of the lac operon. Contrary to expectation, with certain pairs of mutants this enzyme, upon dissociation, does not yield two independent polypeptides but only a single continuous protomer. Both genetic recombination and suppression have been ruled out as the source of this phenomenon. Therefore we believe we are observing the synthesis of one protein from two independently functioning genes.
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