Open AccessSynthesis of Proteochondroitin Sulfate by Normal, Nanomelic, and 5-Bromodeoxyuridine-Treated Chondrocytes in Cell Culture
Author(s) -
Marshall J. Palmoski,
Paul F. Goetinck
Publication year - 1972
Publication title -
proceedings of the national academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.69.11.3385
Subject(s) - bromodeoxyuridine , cell culture , embryonic stem cell , chemistry , sulfate , cell , microbiology and biotechnology , biochemistry , biology , cell growth , genetics , organic chemistry , gene
Chondrocytes grown in cell culture from micromelic chick sterna and 5-bromodeoxyuridine-treated normal cells showed reduced incorporation of sulfate into hyaluronidase-sensitive material as compared to normal chondrocytes. Proteochondroitin sulfate synthesized by normal embryonic chick chondrocytes in cell culture was heterogeneous on chromatography. The major chromatographic component was specifically inhibited in normal chondrocytes treated with 5-bromodeoxyuridine, and this component was absent in nanomelic chondrocytes.
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