Open AccessSpecificity of the Break Produced by Restricting Endonuclease R 1 in Simian Virus 40 DNA, as Revealed by Partial Denaturation Mapping
Author(s) -
Carel Mulder,
Hajo Delius
Publication year - 1972
Publication title -
proceedings of the national academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.69.11.3215
Subject(s) - dna , denaturation (fissile materials) , nucleic acid denaturation , endonuclease , restriction enzyme , molecule , chemistry , nucleic acid thermodynamics , escherichia coli , biology , microbiology and biotechnology , biophysics , crystallography , biochemistry , gene , nuclear chemistry , organic chemistry , base sequence
Superhelical circular (form I) SV40 DNA was converted to linear molecules by the action of a partially purified restriction enzyme of Resistance Transfer Factor-R(1) of Escherichia coli. The resulting linear DNA molecules are full length, as judged by their sedimentation through alkaline sucrose gradient and by direct observation in an electron microscope. Nicked circular (form II) DNA was found as an intermediate in the conversion of form I DNA to linear DNA. Analysis of partial denaturation maps obtained by alkaline denaturation of the unitlength linear molecules showed that the break in SV40 DNA occurred at a specific site on the DNA.
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