Open AccessIn Vivo Production of an RNA-DNA Copolymer after Infection of Escherichia coli by Bacteriophage T4
Author(s) -
Patrick J. Buckley,
Linda D. Kosturko,
Andrzej W. Kozinski
Publication year - 1972
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.69.11.3165
Subject(s) - bacteriophage , polynucleotide , escherichia coli , dna , rna , biology , microbiology and biotechnology , dna replication , chemistry , biochemistry , gene
An RNA-DNA copolymer was isolated fromEscherichia coli infected with bacteriophage T4. The RNA and DNA are covalently linked, and in the same polynucleotide strand. The DNA of the copolymer hybridizes specifically to the left strand of phage T4 DNA. The copolymer is produced in cells infected with amber mutants of phage T4 deficient in DNA replication and is not inhibited by the addition of chloramphenicol.