Open AccessMammalian Cells with Altered Forms of RNA Polymerase II
Author(s) -
Vivien Chan,
G. F. Whitmore,
Louis Siminovitch
Publication year - 1972
Publication title -
proceedings of the national academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.69.11.3119
Subject(s) - chinese hamster ovary cell , mutant , polymerase , biology , microbiology and biotechnology , rna polymerase , rna polymerase ii , gene , rna polymerase iii , mutation , rna , genetics , rna dependent rna polymerase , gene expression , cell culture , promoter
Mutants of Chinese hamster ovary cells that are resistant to α-amanitin can be isolated. At least some of these mutants contain an altered form of DNA-dependent RNA polymerase II, as indicated by its resistance to α-amanitin. These results indicate that mutation to α-amanitin resistance involves a change of a structural gene.
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