Open AccessEvidence for the Existence of Several Molecular Species in the “45S Fraction” of Mammalian Ribosomal Precursor RNA
Author(s) -
Pierre Tiollais,
Francis Galibert,
M Boiron
Publication year - 1971
Publication title -
proceedings of the national academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.68.6.1117
Subject(s) - ribosomal rna , rna , 5.8s ribosomal rna , 5s ribosomal rna , uridine , homogeneous , biology , small nuclear rna , microbiology and biotechnology , biochemistry , non coding rna , chemistry , ribosome , gene , physics , thermodynamics
Analysis of RNA (after 5 or 60 min of labeling with [(3)H]uridine) from L5178Y cells by electrophoresis in 1.7% polyacrylamide gels demonstrates that the ribosomal RNA "45S fraction" is not homogeneous but consists of at least three molecular species, conventionally designated 47S RNA, 46S RNA, and 45S RNA. Alternatives to the classical scheme for the biosynthesis of ribosomal RNA in mammalian cells are discussed.
Accelerating Research
Robert Robinson Avenue,
Oxford Science Park, Oxford
OX4 4GP, United Kingdom
Address
John Eccles HouseRobert Robinson Avenue,
Oxford Science Park, Oxford
OX4 4GP, United Kingdom