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The replication of the ring-shaped polyoma DNA has been investigated. The replicative intermediate has been identified and characterized; it consists of molecules containing both circular and nicked strands, not longer than the strands of the DNA found in viral particles. The findings exclude certain proposed methods of replication of ring-shaped DNA and are compatible with Cairns' model.

THE REPLICATION OF THE RING-SHAPED DNA OF POLYOMA VIRUS, I. IDENTIFICATION OF THE REPLICATIVE INTERMEDIATE