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The transcription process can in theory be used for genetic mapping on the "intraoperonal" level. We have shown that such an approach, utilizing actinomycin D to create blocks to the travel of the transcription enzyme, is indeed feasible, and the effects of actinomycin D on the synthesis of general pulse-labeled RNA are reported herein.

“TRANSCRIPTIONAL MAPPING,” I. INTRODUCTION TO THE METHOD AND THE USE OF ACTINOMYCIN D AS A TRANSCRIPTIONAL MAPPING AGENT