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to; those which develop in conditioned medium. By quantitative criteria also (percentage of muscle colonies which develop) the use of collagen substratum replaces the requirement for conditioned medium. The implication of these results is that the development of muscle cells requires the presence of a metabolic product of an associated cell type-the fibroblast. The development of a number of different cell types has been shown to be dependent upon the close proximity of connective tissue elements. It is not unlikely that collagen plays a common role in a variety of differentiative events.

Individuality in nutrition: effects of vitamin A-deficient and other deficient diets on experimental animals.