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18 Although large numbers of ribosomes sediment with nuclei and mitochondria, most of these ribosomes are not actually bound to these structures. Indeed, if nuclei and over 95% of the mitochondria are removed by centrifuging at 8500 grnax for 10 min,9 considerable quantities of ribosomes whose size distribution is nearly identical to that of those from Fractions I and II can be recovered in the pellet obtained by further centrifugation at the speed conventionally used to prepare the postmitochondrial supernatant fraction (20,000 gmax for 20 min). 19 Schneider, W. C., and G. H. Hogeboom, J. Biol. Chem., 183, 123 (1950). 20 Britten, R. J., and R. B. Roberts, Science, 131, 32 (1960). 21 Increasing the concentration of ribosomes layered onto the gradient did not substantially change the pattern of polyribosome size distribution but did result in some loss of resolution of the individual peaks.

MEIOTIC CONJUNCTIVE ELEMENTS NOT INVOLVING CHIASMATA