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After the poly-U experiments, the next major challenge was finding out what the remaining codewords were. In this article, the authors use a rapid method for measuring sRNA binding to ribosomes and try to resolve some problems with ambiguous and synonymous codewords. The experimental evidence suggests the binding of specific types of sRNA to ribosomes is directed by only certain corresponding trinucleotides, making sequencing efforts more feasible in the coding process.

RNA CODEWORDS AND PROTEIN SYNTHESIS, II. NUCLEOTIDE SEQUENCE OF A VALINE RNA CODEWORD