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In all previous in vitro experiments3'antibody formation could be demonstrated only because of the high potency of the antigens used and because of the very sensitive immunological methods available for the detection of antibodies against particulate antigens and toxins. The method of incubation described here would seem to hold promise for other experiments requiring the maintenance of differentiated cells in a functional state in vitro.

PRODUCTION OF PROTOPLASTS OF ESCHERICHIA COLI BY LYSOZYME TREATMENT