English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Significance G protein–coupled receptors (GPCRs) have been proposed to signal in multiple compartments within the cell, a phenomenon that could have important implications for our understanding of receptor pharmacology and disease treatment. However, directly measuring G protein activity in subcellular compartments remains a challenge, and the mechanism underlying their translocation and activation remains elusive. Using a biosensor that specifically measures the presence of GTP-loaded Gq/11 at endosomes, our findings reveal the mechanism underlying Gq trafficking and signaling in the endosome and provide a powerful tool to directly monitor G protein movement and activation in subcellular compartments.

BRET-based effector membrane translocation assay monitors GPCR-promoted and endocytosis-mediated G q activation at early endosomes

BRET-based effector membrane translocation assay monitors GPCR-promoted and endocytosis-mediated G _q activation at early endosomes