Open AccessEpigenetic competition reveals density-dependent regulation and target site plasticity of phosphorothioate epigenetics in bacteria
Author(s) -
Xiaolin Wu,
Bo Cao,
Patricia Aquino,
Tsu-Pei Chiu,
Chao Chen,
Susu Jiang,
Zixin Deng,
Shi Chen,
Remo Rohs,
Lianrong Wang,
James E. Galagan,
Peter C. Dedon
Publication year - 2020
Publication title -
proceedings of the national academy of sciences
Language(s) - English
Resource type - Journals
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.2002933117
Subject(s) - epigenetics , biology , genome , dna , genetics , bacterial genome size , computational biology , dna methylation , consensus sequence , methyltransferase , dna sequencing , gene , methylation , gene expression , base sequence
Significance The significance of this work lies in the application of innovative analytical, sequencing, and computational tools to discover a novel epigenetic regulatory mechanism. Here, we discovered that phosphorothioate (PT) DNA modifications are maintained at a constant density in a genome in part by DNA shape-driven target site selection. While structurally similar GAAC, GTTC, and GATC motifs are all modified by PT-catalyzing proteins, methylation of G6m ATC by Dam methyltransferase blocks PT modification of GATC and shifts PTs to the other two sites inSalmonella enterica , maintaining a constant number of PTs in the genome.
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