Open AccessInvariable stoichiometry of ribosomal proteins in mouse brain tissues with aging
Author(s) -
Susan Amirbeigiarab,
Parnian Kiani,
Ana K. Velazquez Sanchez,
Christoph Krisp,
Andriy Kazantsev,
Lars Fester,
Hartmut Schlüter,
Zoya Ignatova
Publication year - 2019
Publication title -
proceedings of the national academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.1912060116
Subject(s) - ribosome , biology , ribosomal rna , ribosomal protein , ribosome profiling , eukaryotic ribosome , polysome , biochemistry , microbiology and biotechnology , rna , gene
Significance Ribosomes—the universal biosynthesis machinery central to all cellular life—are composed of 4 ribosomal RNA (rRNA) and up to 80 ribosomal proteins (RPs), yet detecting fluctuations in their RP composition with possible functional consequences remains a challenge. Using quantitative proteomics we show an invariant RP composition of ribosomes across 3 murine brain and liver tissues. The RP pool does not change with age and remains constant in tissues of juvenile, adult, and middle-aged mice groups. In a broader context, the high robustness in the overall protein architecture of the ribosomes across tissues and ages implies that ribosomal heterogeneity, if any, may result from other processes, including postsynthesis modifications of rRNA and RPs.
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