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Significance The insect-disseminated bacteriumCandidatus Liberibacter asiaticus causes the destructive, incurable “citrus greening disease,” which is widespread in Asia, Africa, and the Americas, resulting in economic losses in the billions of dollars. One approach to treating infected citrus trees is application of antimicrobial compounds. However, use of broad-spectrum antibiotics in commercial citrus orchards has significant disadvantages, such as emergence of resistance and inhibition of beneficial bacteria. We designed a synthetic system for high-throughput screening of compound libraries in a closely related, culturable, and nonpathogenic bacterium, allowing us to identify small molecule inhibitors ofCa . L. asiaticus transcription activators.

A high-throughput system to identify inhibitors of Candidatus Liberibacter asiaticus transcription regulators