Open AccessChromatin conformation remains stable upon extensive transcriptional changes driven by heat shock
Author(s) -
Judhajeet Ray,
Paul R. Munn,
Anniina Vihervaara,
James J. Lewis,
Abdullah Ozer,
Charles G. Danko,
John T. Lis
Publication year - 2019
Publication title -
proceedings of the national academy of sciences
Language(s) - English
Resource type - Journals
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.1901244116
Subject(s) - hsf1 , chromatin , enhancer , heat shock factor , nucleosome , promoter , biology , chromatin immunoprecipitation , heat shock , microbiology and biotechnology , gene , genetics , transcription (linguistics) , transcription factor , heat shock protein , gene expression , hsp70 , philosophy , linguistics
Significance Dramatic and rapid changes in transcription take place upon heat shock (HS), where thousands of genes have been shown to be immediately up- or down-regulated in metazoans. The role of large-scale chromatin conformation and changes in long-range interactions between distal regulatory elements and HS-regulated promoters remains unclear. Our study shows that topologically associating domains and compartment structures remain remarkably unchanged upon acute HS in human andDrosophila , while only modest changes of distal regulatory interactions are observed in human cells. These results suggest that the global chromatin structure required for the HS response is preestablished across metazoans in order to drive transcriptional changes in HS responsive genes.
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