English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Significance Chemical cross-linking mass spectrometry is a powerful method to identify protein interaction partners. The cross-links also provide approximate interresidue distances, which can help model the structures of complexes. However, current cross-linking reagents react with only a limited set of amino acid residues and their very high reactivity can induce cross-links between sites that are distant in the native state of the interrogated proteins. We therefore developed a “plant-and-cast” strategy, in which a heterobifunctional cross-linker is “planted” onto surface Lys residues using a highly reactive succinimide ester. The half-reacted cross-linker then “casts” a much less reactive sulfonyl fluoride across the proximal surface resulting in cross-links to neighboring Ser, Thr, Tyr, His, or Lys sidechains in a proximity-enhanced reaction.

Proximity-enhanced SuFEx chemical cross-linker for specific and multitargeting cross-linking mass spectrometry