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Significance While biological utilization of one-carbon (C1) compounds has attracted much attention, previous studies have focused mainly on the utilization of CO2 . Here, we report development ofEscherichia coli strains capable of assimilating formic acid (FA) and CO2 through the C1 assimilation pathway, synthesizing pyruvate from FA and CO2 by establishing the reconstructed tetrahydrofolate cycle and the reverse glycine cleavage pathway. To generate energy and redox while using less glucose, a heterologous formate dehydrogenase was introduced together with the C1 assimilation pathway. The resulting strain could utilize FA and CO2 as sole carbon sources for sustaining growth. This work demonstrates that the combined use of the C1 assimilation pathway and formate dehydrogenase allowsE. coli to utilize FA and CO2 efficiently.

Assimilation of formic acid and CO 2 by engineered Escherichia coli equipped with reconstructed one-carbon assimilation pathways

Assimilation of formic acid and CO ₂ by engineered Escherichia coli equipped with reconstructed one-carbon assimilation pathways