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The different parts of the renal nephron have different functions that are related to reabsorption or secretion processes. This is mediated by genes that show a distinct expression pattern along the renal nephron segments (1). The majority of the segments contain only one epithelial cell type. However, the collecting duct is built up by principal cells (PCs), type A intercalated cells (A-ICs), and type B intercalated cells (B-ICs). Chen et al. (2) applied RNA sequencing on the single-cell level using enriched PCs, A-ICs, and B-ICs. These data clearly show major differences in the transcriptomes of the different cell types. Besides known genes, several other transcripts are … [↵][1]2Email: bayram.edemir{at}uk-halle.de. [1]: #xref-corresp-1-1

Considering hypertonicity in the interpretation and analysis of cell type-specific gene expression pattern in the collecting duct