English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Significance Adaptive immunity against foreign DNA elements in microbes, conferred by their CRISPR systems, has been repurposed for wide-ranging genome-engineering applications. The CRISPR-Cas9 system has been the most widely used system. But to overcome some of its major limitations, the search for novel CRISPR enzymes has been a significant area of interest. One such search led to the discovery of CRISPR-Cpf1 systems. We used single-molecule fluorescence analysis and biochemical assays to characterize the kinetics, specificity, and mechanism of Cpf1’s DNA interrogation, cleavage, and cleavage-product release. Some major differences from Cas9 were observed which will help the use of Cpf1 in areas outside the realm of Cas9 and effectively broaden the scope of CRISPR-based applications.

Real-time observation of DNA target interrogation and product release by the RNA-guided endonuclease CRISPR Cpf1 (Cas12a)