Open AccessVoltage-sensitive rhodol with enhanced two-photon brightness
Author(s) -
Rishikesh U. Kulkarni,
Daniel J. Kramer,
Narges Pourmandi,
Kaveh Karbasi,
Helen S. Bateup,
Evan W. Miller
Publication year - 2017
Publication title -
proceedings of the national academy of sciences
Language(s) - English
Resource type - Journals
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.1610791114
Subject(s) - brightness , fluorescence , membrane potential , sensitivity (control systems) , neuroscience , photon , two photon excitation microscopy , voltage , premovement neuronal activity , biophysics , biological system , optoelectronics , physics , biology , optics , electronic engineering , engineering , quantum mechanics
Significance Fast changes in membrane potential drive the unique physiology of neurons. Despite the critical importance of coordinated neuronal firing, observing neuronal activity in a noninvasive, highly parallel manner remains an outstanding challenge, due in part to a lack of tools that can report on fast changes in membrane potential with sufficient speed, sensitivity, and brightness. We report an optical voltage reporter based on a fluorescent rhodol that shows improved photostability and brightness under both one- and two-photon illumination. We use the indicator RhodolVoltageFluor-5 to probe dynamics of neuronal excitability in a mouse model of genetic epilepsy, both in culture and in brain slices.
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