English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Tools annual

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Presents the access of premium content as premium feature

Premium Content

Global: Zendy Plus annual

Global: Zendy Free Plan

Significance Sperm capacitation enables spermatozoa to undergo the acrosome reaction and to exhibit vigorous motility called hyperactivation. At the molecular level, capacitation is associated with activation of a cAMP-dependent pathway and with the increase of intracellular pH and Ca2+ concentrations. Ca2+ ionophore A23187 elevates intracellular Ca2+ and induces the acrosome reaction but renders the spermatozoa motionless. However, when the ionophore was washed away, spermatozoa recovered motility, showed hyperactivation, and were able to fertilize cumulus-intact eggs. In these conditions, sperm acquired fertilizing capacity even when the cAMP pathway was inactivated. Fertilized oocytes with A23187-treated sperm developed into normal offspring. These data indicate that a short elevation of intracellular Ca2+ overcomes other necessary signaling pathways during capacitation and renders sperm fertile.

proceedings of the national academy of sciences of the united states of america

Ca <sup>2+</sup> ionophore A23187 can make mouse spermatozoa capable of fertilizing in vitro without activation of cAMP-dependent phosphorylation pathways

Ca 2+ ionophore A23187 can make mouse spermatozoa capable of fertilizing in vitro without activation of cAMP-dependent phosphorylation pathways

Ca ²⁺ ionophore A23187 can make mouse spermatozoa capable of fertilizing in vitro without activation of cAMP-dependent phosphorylation pathways