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Our aerobiology team was pleased to read “Microbiome of the upper troposphere: Species composition and prevalence, effects of tropical storms, and atmospheric implications” by DeLeon-Rodriguez et al. (1). On the opposite side of the continent, at a different time of year, and using other molecular methods, we also found rich bacterial assemblages in the upper troposphere capable of long-range transport (2, 3). We were delighted to see the convergence and hope it will inspire follow-up investigations because the field truly needs a global monitoring network to enhance understanding of patterns and implications. However, we have some concerns about the work by DeLeon-Rodriguez et al. (1). First, the Materials and Methods (both in the article and SI Materials and Methods) of DeLeon-Rodriguez et …

Inadequate methods and questionable conclusions in atmospheric life study