English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Transcription activator-like effector (TALE) proteins of the plant pathogenic bacterial genusXanthomonas bind to and transcriptionally activate host susceptibility genes, promoting disease. Plant immune systems have taken advantage of this mechanism by evolving TALE binding sites upstream of resistance (R ) genes. For example, the pepperBs3 and riceXa27 genes are hypersensitive reaction plantR genes that are transcriptionally activated by corresponding TALEs. BothR genes have a hallmark expression pattern in which their transcripts are detectable only in the presence and not the absence of the corresponding TALE. By transcriptome profiling using next-generation sequencing (RNA-seq), we tested whether we could avoid laborious positional cloning for the isolation of TALE-inducedR genes. In a proof-of-principle experiment, RNA-seq was used to identify a candidate forBs4C , anR gene from pepper that mediates recognition of theXanthomonas TALE protein AvrBs4. We identified one majorBs4C candidate transcript by RNA-seq that was expressed exclusively in the presence of AvrBs4. Complementation studies confirmed that the candidate corresponds to theBs4C gene and that an AvrBs4 binding site in theBs4C promoter directs its transcriptional activation. Comparison ofBs4C with a nonfunctional allele that is unable to recognize AvrBs4 revealed a 2-bp polymorphism within the TALE binding site of theBs4C promoter.Bs4C encodes a structurally unique R protein andBs4C -like genes that are present in many solanaceous genomes seem to be as tightly regulated as pepperBs4C . These findings demonstrate that TALE-specificR genes can be cloned from large-genome crops with a highly efficient RNA-seq approach.

RNA-seq pinpoints a Xanthomonas TAL-effector activated resistance gene in a large-crop genome