English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Ion homeostasis is a fundamental cellular process particularly important in excitable cell activities such as hearing. It relies on the Na+ /K+ ATPase (also referred to as the Na pump), which is composed of a catalytic α subunit and a β subunit required for its transport to the plasma membrane and for regulating its activity. We show that α and β subunits are expressed in Johnston's organ (JO), theDrosophila auditory organ. We knocked down expression of α subunits (ATPα andα-like ) and β subunits(nrv1, nrv2 , andnrv3) individually in JO with UAS/Gal4-mediated RNAi. ATPα shows elevated expression in the ablumenal membrane of scolopale cells, which enwrap JO neuronal dendrites in endolymph-like compartments. Knocking downATPα , but notα-like , in the entire JO or only in scolopale cells using specific drivers, resulted in complete deafness. Among β subunits,nrv2 is expressed in scolopale cells andnrv3 in JO neurons. Knocking downnrv2 in scolopale cells blocked Nrv2 expression, reduced ATPα expression in the scolopale cells, and caused almost complete deafness. Furthermore, knockdown of eithernrv2 orATPα specifically in scolopale cells causes abnormal, electron-dense material accumulation in the scolopale space. Similarly,nrv3 functions in JO but not in scolopale cells, suggesting neuron specificity that parallelsnrv2 scolopale cell–specific support of the catalytic ATPα. Our studies provide an amenable model to investigate generation of endolymph-like extracellular compartments.

Cell-type–specific roles of Na + /K + ATPase subunits in Drosophila auditory mechanosensation

Cell-type–specific roles of Na ⁺ /K ⁺ ATPase subunits in Drosophila auditory mechanosensation