English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

The availability of facile methods for targeted gene knockout and gene replacement based on homologous recombination in bacteria and yeast systems has driven rapid progress in understanding many of the complex metabolic and regulatory networks in prokaryotic and eukaryotic cells. The lack of such tools in other organisms is a major impediment to progress both in fundamental research and in research that is directed toward practical economic and societal outcomes. For example, the current goal to replace fossil fuels with renewable energy sources produced by plants and algae is hampered in both the short term and the long term by our limited knowledge of their metabolic systems and how they can be modified to create organisms that produce more and better energy-rich molecules. Thus, the report in PNAS by Kilian et al. (1) of efficient and reliable genetic transformation of the commercially important alga, Nannochloropsis, via homologous recombination is a significant step forward. Nannochloropsis species have been used for several decades to produce nutraceuticals and feed supplements (2⇓⇓–5). Several Nannochloropsis species have received attention more recently as oil-rich organisms with promise as a source of algal biofuels (6⇓⇓–9). Nonetheless, relatively little is known about the biology of this algal species, including the genes and enzymes involved in lipid biosynthesis. This situation is changing rapidly with the advent of important new tools, not the least of which is the ability to perform targeted gene knockouts and gene replacements using the methods of Kilian et al. (1). Coupled with …

Homologous recombination in Nannochloropsis : A powerful tool in an industrially relevant alga