Open AccessTranscription coactivator SAYP combines chromatin remodeler Brahma and transcription initiation factor TFIID into a single supercomplex
Author(s) -
Nadezhda E. Vorobyeva,
Н. В. Сошникова,
J. V. Nikolenko,
Julia L. Kuzmina,
Е. Н. Набирочкина,
С. Г. Георгиева,
Yulii V. Shidlovskii
Publication year - 2009
Publication title -
proceedings of the national academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.0901801106
Subject(s) - transcription factor ii a , transcription factor ii d , coactivator , general transcription factor , transcription factor ii f , taf2 , transcription preinitiation complex , rna polymerase ii , eukaryotic transcription , taf1 , rna polymerase ii holoenzyme , microbiology and biotechnology , genetics , biology , transcription factor , promoter , enhancer , transcriptional regulation , gene expression , gene
Transcription activation by RNA polymerase II is a complicated process driven by combined, precisely coordinated action of a wide array of coactivator complexes, which carry out chromatin-directed activities and nucleate the assembly of the preinitiation complex on the promoter. Using various techniques, we have shown the existence of a stable coactivator supercomplex consisting of the chromatin-remodeling factor Brahma (SWI/SNF) and the transcription initiation factor TFIID, named BTFly (Brahma and TFIID in one assembly). The coupling of Brahma and TFIID is mediated by the SAYP factor, whose evolutionarily conserved activation domain SAY can directly bind to both BAP170 subunit of Brahma and TAF5 subunit of TFIID. The integrity of BTFly is crucial for its ability to activate transcription. BTFly is distributed genome-wide and appears to be a means of effective transcription activation.
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