Open AccessDNA repair capacity of zebrafish
Author(s) -
Raquel Sussman
Publication year - 2007
Publication title -
proceedings of the national academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.0706157104
Subject(s) - zebrafish , danio , luciferase , creatures , biology , dna , dna repair , plasmid , luciferases , grossman , population , gene , reporter gene , microbiology and biotechnology , computational biology , genome , genetics , gene expression , transfection , medicine , keynesian economics , paleontology , economics , environmental health , natural (archaeology)
Damage to the genome is unavoidable in living creatures, because of sunlight exposure as well as environmental chemicals present in food and drinking water. There is a need to monitor and purify the drinking water; therefore, several methods of detection have been developed. A very promising model system for this purpose is the zebrafish (Danio rerio), which is endowed with special qualities for detecting external as well as internal abnormalities. Grossman and Wei's assay [Grossman L, Wei Q (1995) Clin Chem 12:1854-1863], which measures the expression level of a nonreplicating recombinant plasmid DNA containing a UV-damaged luciferase reporter gene, shows that zebrafish can repair chromosomal lesions to a much greater extent than the human population. This vertebrate model is still very promising after possible down-regulation of the DNA repair enzymes.
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